Name / Stock Number: pDamBOB_DAM-GR-EV / CD3-2939

donor stock number: pDamBOB_DAM-GR-EV

Resource Type: plasmid

Availability: available

Donors:
  • Matthew Brooks
  • Gloria Coruzzi
  • Jose Alvarez

Donation Date: 01/17/2023

Date Released: 02/07/2023

Description:

Plasmid for transient DamID (DNA adenine methyltransferase identification) in Arabidopsis protoplasts. Expresses a Dam-GR fusion that serves as an empty vector (no TF) transfection control to measure background DNA methylation from a Dam unguided by a TF. Must be maintained in PIR1 cells.

Growth Requirement: grow in LB media at 37C overnight

Marker: ampicillin

Background:

ABRC Comment:

Format Shipped: bacterial stab

Base / Commercial Price: $15 / $120

Plasmid

Promoter:

Reporter: mCherry

Type: construct

E. coli PIR1

Additional Information

DOI PubMed
10.1038/s41467-020-14979-6 32123177

Quality Control Comments

There is no quality control data for this stock.