donor stock number: pDamBOB_DAM-GR-NLP7
Resource Type: plasmid
Availability: available
Donors:- Matthew Brooks
- Gloria Coruzzi
- Jose Alvarez
Donation Date: 01/17/2023
Date Released: 02/07/2023
Description:
Plasmid for transient DamID (DNA adenine methyltransferase identification) in Arabidopsis protoplasts. Expresses a Dam-GR-NLP7 fusion for DamID. The NLP7 DNA sequence has been optimized to remove GATC sequences while maintaining the NLP7 amino acid sequence. Plasmid forms plasmid dimers in vivo. Must be maintained in PIR1 cells.
Growth Requirement: grow in LB media at 37C overnight
Marker: ampicillin
Background:
ABRC Comment:
Format Shipped: bacterial stab
Base / Commercial Price: $15 / $120
Plasmid
Promoter:
Reporter: mCherry
Type: construct
E. coli PIR1
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/s41467-020-14979-6 | 32123177 |
Quality Control Comments
There is no quality control data for this stock.