Name / Stock Number: RUBY-ABE2 / CD3-2961

other name: pYPQ5237

Resource Type: plasmid

Availability: available

Is restricted: true

Donors:
  • Yiping Qi

Donation Date: 01/22/2025

Date Released: 01/24/2025

Description:

E coli strain harboring a binary all-in-one expressing vector (pLR3850) containing the expression cassettes for the sgRNA matching RUBYm2 and the Cas9 nickase (nCas9D10A) translationally fused with the engineered adenosine deaminase ecTadA8e, resulting in a functional adenine base editor (ABE2)

Growth Requirement: grow in LB media at 37C

Marker: kanamycin

Background:

ABRC Comment:

Format Shipped: bacterial stab culture

Base / Commercial Price: $15 / $120

Plasmid

Promoter:

Reporter:

Type: construct

E. coli DH5-alpha

Additional Information

Quality Control Comments

There is no quality control data for this stock.

Note

Yunde Zhao RUBY reporter system

PLEASE BE AWARE this stock should not be received by or transmitted to any private company. As an academic researcher, you are not restricted in the use of any findings developed from these stocks, but the stocks themselves are for the use of academic researchers only. If you are an employee of a company and we have shipped the seeds to you in error, please contact us immediately at abrc@osu.edu.