Name / Stock Number: Walker Two-hybrid cDNA library / CD4-10

Stock Type: pool

Resource Type: library

Availability: available

Donors:
  • John Walker

Donation Date: 03/31/1995

Date Released: 09/26/1996

Description:

Yeast two-hybrid cDNA library in LAMBDA-ACT vector; generated from Nossen (No-0) random-primed mRNA; yeast strains Y190 and Y190/SNF1/SNF4, bacterial strains BNN132 and LE392, and plasmids PAS1 and PAS1/CHY2 for two hybrid protocol are shipped with the library; cloning enzymes: XhoI and XhoI

Growth Requirement:

Marker: ampicillin

Background: No (Nossen)

ABRC Comment:

Format Shipped: pooled library shipped frozen in 7% DMSO; yeast strains Y190 and Y190SNF1/SNF4 shipped as slant cultures; bacterial strains BNN132 and LE392, and plasmids PAS1 and PAS1/CHY2 shipped as stab cultures

Express Shipping Required: true

Base / Commercial Price: $15 / $120

Arabidopsis thaliana 3702

Additional Information

CD4-10 instructions

Quality Control Comments

Comment Commented by Date