ABRC
Stock Type: pool
Resource Type: library
Availability: not distributed, ABRC inventory of this stock has been exhausted and will no longer be distributed.
Donors:- Athanasios Theologis
Donation Date: 06/21/1996
Date Released: 01/19/1996
Description:
Yeast two-hybrid cDNA library in LAMBDA-ACT vector; generated from Columbia (Col) mRNA isolated from 3 day-old etiolated seedlings; library consists of 36,000,000 independent recombinants and was amplified on LE392; 22 of 23 clones tested contained inserts; yeast strains Y190 and Y190SNF1/SNF2, bacterial strain BNN132, and plasmids PAS1 and PAS1/CHY2 for two hybrid protocol are shipped with the library; cloning enzymes: XhoI and XhoI
Growth Requirement:
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: pooled library shipped frozen in 7% DMSO; yeast strains shipped as slant cultures; bacterial strain and plasmids shipped as stab cultures
Express Shipping Required: true
Base / Commercial Price: $15 / $120
Taxon: Arabidopsis thaliana 3702
Quality Control Comments
There is no quality control data for this stock.