Arabidopsis Biological Resource Center

Description:

Transgenic line tagged with fluorescent proteins. Nuclei, plasma membranes, mitochondria, and plastids are genetically tagged with CFP, GFP, YFP, and RFP respectively. Agrobacterium tumefaciens strain GV3101/pMP90 was transformed with the vectors, pKW102-CY-MODC, pEL103-RecA::DsRed2 , and pEL103-GR respectively. These Agrobacterium strains were then used to transform Arabidopsis thaliana (Columbia accession). Zygosities of each transgenic line were determined by their mortalities on kanamycin selection plates (50 ug/ mL) and by microscopic detections of the fluorescent proteins in their seedlings. Homozygous lines of transgenic plants expressing Gal4-CFP and CoxIV-YFP were crossed to homozygous lines of transgenic plants expressing Cam53BD-GFP. The resulting homozygous lines were further crossed to homozygous transgenic plants expressing RecA-RFP to obtain the Kaleidocell lines. The line that most strongly expressed all transgenes was designated Kaleidocell.17. Kaleidocell.17 was self-pollinated in the following three generations. The line that expressed all transgenes in its all progenies was identified and designated Kaleidocell.17.1.7. The progenies of Kaleidocell.17.1.7 were used as the homozygous of Kaleidocell. The second progenies of Kaleidocell.17.1.7 (designated Kalediocell.17.1.7.7.X) are the distribution stock.

Growth Requirement: none

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120