donor stock number: wei8-1 tar1-1 tar2-1/+ DR5:GUS
NASC stock number: N16419
Resource Type: seed
Availability: available
Donors:- Anna Stepanova
- Jose Alonso
Donation Date: 02/27/2009
Date Released: 03/05/2009
Description:
Triple mutant generated by crossing sesquimutant wei8-1 tar2-1/+ with homozygous tar1-1; DR5:GUS reporter was introgressed into mutant background by crossing.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Segregates ~1/4 of monopteros-like seedlings (wei8-1 tar1-1 tar2-1 triple mutant plants), ~1/2 of seedlings with moderately ethylene-insensitive roots after 3 days in the dark (wei8-1 tar1-1 tar2-1/+), and ~1/4 of weakly ethylene insensitive seedlings (wei8-1 tar1-1 double mutant plants); triple mutant plants do not survive on soil.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1016/j.cell.2008.01.047 | 18394997 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pSKI15 | activation tag T-DNA | 4x CaMV 35S | BASTA | |
| pGABI1 | T-DNA | sulfadiazine | ||
| DR5:GUS | promoter reporter construct | DR5 | GUS | |
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
There is no quality control data for this stock.