Name / Stock Number: Gibberellic Acid Physiology and Plant Transformation / CS16562

NASC stock number: N16562

Stock Type: set

Resource Type: education kit

Availability: available

Donors:
  • John Ohlrogge
  • Maria Molina

Donation Date: 02/08/2011

Date Released: 02/08/2011

Description:

This set of three seed stocks and two DNA stocks can be used with the "Gibberellic Acid Physiology and Plant Transformation" module to introduce GA (gibberellic acid) hormone physiology and plant transformation in an undergraduate laboratory module. In this module, Arabidopsis ga5 semi-dwarf mutant plants with reduced activity of GA 20-oxidase are transformed with a construct containing the GA5 gene. The dwarf mutant plants transformed with a wild-type version of the gene show normal size, demonstrating successful complementation of the mutant phenotype. Besides visual observation of the phenotype, students learn additional techniques, including PCR and agarose gel electrophoresis. This experiment is cost effective and can be completed within a 4-month term.

Growth Requirement: none

Marker:

Background:

ABRC Comment: Order this set only if approved to work with transgenic material. The set does not include the Agrobacterium strain needed for transformation.

Format Shipped: 100 seeds per vial, plasmids as bacterial stab cultures

Base / Commercial Price: $35 / $280

Education Kit

TRAINED
Hormone Physiology, Molecular Biology
College/University

Arabidopsis thaliana 3702

Additional Information

Quality Control Comments

There is no quality control data for this stock.

Note

TRAINED_transgenic

This education kit contains transgenic seeds. To be eligible to order these stocks, you must confirm that you are able to dispose of the plant material following safety protocols prescribed for a biosafety level 1 facility at your school, or in partnership with a nearby laboratory.