Name / Stock Number: Gibberellic Acid Physiology and Plant Transformation / CS16562
NASC stock number: N16562
Stock Type: set
Resource Type: education kit
Availability: available
Donors:- John Ohlrogge
- Maria Molina
Donation Date: 02/08/2011
Date Released: 02/08/2011
Description:
This set of three seed stocks and two DNA stocks can be used with the "Gibberellic Acid Physiology and Plant Transformation" module to introduce GA (gibberellic acid) hormone physiology and plant transformation in an undergraduate laboratory module. In this module, Arabidopsis ga5 semi-dwarf mutant plants with reduced activity of GA 20-oxidase are transformed with a construct containing the GA5 gene. The dwarf mutant plants transformed with a wild-type version of the gene show normal size, demonstrating successful complementation of the mutant phenotype. Besides visual observation of the phenotype, students learn additional techniques, including PCR and agarose gel electrophoresis. This experiment is cost effective and can be completed within a 4-month term.
Growth Requirement: none
Marker:
Background:
ABRC Comment: Order this set only if approved to work with transgenic material. The set does not include the Agrobacterium strain needed for transformation.
Format Shipped: 100 seeds per vial, plasmids as bacterial stab cultures
Base / Commercial Price: $35 / $280
Education Kit
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1002/bmb.20263 | 21567727 |
Quality Control Comments
There is no quality control data for this stock.
Note
TRAINED_transgenicThis education kit contains transgenic seeds. To be eligible to order these stocks, you must confirm that you are able to dispose of the plant material following safety protocols prescribed for a biosafety level 1 facility at your school, or in partnership with a nearby laboratory.