NASC stock number: N16660
donor stock number: YNACS5YCACS1
Resource Type: seed
Availability: available
Donors:- Atsunari Tsuchisaka
- Athanasios Theologis
Donation Date: 09/10/2009
Date Released: 04/26/2010
Description:
YN (the N-terminus of Citrine YFP with Q69M mutation (Griesbeck et al., J Biol Chem 2001)) and YC (the C-terminus of YFP) were used to make the N-terminal fusion and C-terminal fusion proteins for Bimolecular Fluorescence Complementation (BiFC), respectively. Aside from the T7 tag, YNACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP132 vector. Aside form the His tag, YCACS constructs contained ACS coding sequence and ACS 3' UTR under control of the corresponding ACS promoter in pPZP122 vector. Each N-terminal BiFC construct in pPZP132 vector and each C-terminal BiFC construct in pPZP122 were separately introduced in the pentuple2 mutant acs2-1acs4-1acs5-2acs6-1acs9-1 (CS16644) by transformation. The single identified homozygous line of pentuple 2 mutant transformed with the N-terminal BiFC construct was crossed to the single identified homozygous line of pentuple 2 mutant transformed with the C-terminal BiFC construct.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
phenotype not determined
T-DNA insertion
Arabidopsis thaliana 3702
Additional Information
DOI | PubMed |
---|---|
10.1534/genetics.109.107102 | 19752216 |
Transgene | Description | Promoter | Reporter | Marker |
---|---|---|---|---|
YNACS5 | ACS5 coding sequence and 3' UTR under control of the ACS5 promoter, N-terminus of YFP | |||
YCACS1 | ACS1 coding sequence and 3' UTR under control of the ACS1 promoter, C-terminus of YFP |
Quality Control Comments
There is no quality control data for this stock.