Arabidopsis Biological Resource Center

Description:

This set of two stocks can be used with the "Expression Analysis of Light-regulated Genes in the det1-1 Mutant" module to explore light signaling pathways in plants, specifically Arabidopsis. Over the course of three labs, students are introduced to the techniques of RNA isolation and Reverse Transcription PCR (RT-PCR) for the purpose of analyzing differences in mRNA content of light-regulated genes between wild-type and det1-1 mutant samples. First, students are able to assess phenotypic differences between wild-type and det1-1 plants grown in the presence or absence of light. Next, through RT-PCR students gain an understanding of how mutation in one gene can cause downstream effects in signaling pathways to alter the response to light.

Growth Requirement: none

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Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120