NASC stock number: N2105613
other name: mCITRINE-2xPH(PLC)
donor stock number: P24Y
Resource Type: seed
Availability: available
Donors:- Yvon Jaillais
Donation Date: 10/27/2014
Date Released: 11/13/2014
Description:
PIPline Marker line. Genetically encoded biosensor for PI(4,5)P2 (phosphatidylinositol 4,5-phosphate) is fused with mCITRINE (YFP), cloned into a multisite Gateway destination vector (pB7m34GW), and expressed under control of the UBQ10 promoter. The ligand-binding domain (LBD) that interacts with PI(4,5)P2 is PH; the LBD is from the protein: PLCdelta1 (Rattus norvegicus). Basta resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
mCITRINE (YFP) localization at the plasma membrane
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| UBQ10prom::CITRINE-2xPH(PLCd1) | UBQ10 | mCITRINE | BASTA |
Quality Control Comments
There is no quality control data for this stock.