NASC stock number: N2105624
other name: UBQ10prom::2xCyPet-1xPH(FAPP1)
donor stock number: P5C
Resource Type: seed
Availability: available
Donors:- Yvon Jaillais
Donation Date: 10/27/2014
Date Released: 11/13/2014
Description:
PIPline Marker line. Genetically encoded biosensor for PI4P (phosphatidylinositol 4-phosphate) is fused with 2xCyPET (cyan fluorescent protein), cloned into a multisite Gateway destination vector (pK7m34GW), and expressed under control of the UBQ10 promoter. The ligand-binding domain (LBD) that interacts with PI4P is PH; the LBD is from the protein: FAPP1 (Saccharomyces cerevisiae). Kanamycin resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
2xCyPET localization at the plasma membrane, post-golgi/endosomal compartment (early endosome/TGN + recycling endosome) and weakly in the golgi
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| UBQ10prom::2xCyPet-1xPH(FAPP1) | UBQ10 | CyPet | kanamycin |
Quality Control Comments
There is no quality control data for this stock.