donor stock number: KN::NTF/pK7m34GW
NASC stock number: N2106191
other name: BREAK KN
Resource Type: seed
Availability: available
Donors:- Yvon Jaillais
- Gregory Vert
Donation Date: 11/28/2016
Date Released: 03/03/2017
Description:
BREAK line; Cell type specific promoter expressing a NTF tag in the nuclear envelope for nuclei purification using the INTACT method; Part of the SWELLine collection of markers expressed in specific root tissues for functional genomics in Arabidopsis thaliana; kanamycin selection
Growth Requirement: none
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Temporal expression of GFP in nuclear envelope
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| KN::NTF | KNOLLE | GFP | kanamycin | |
| ACT2:BirA | ACTIN2 promoter: from -1444 to -1 relative to ATG start codon driving the E. coli biotin ligase (BirA) gene, will biotinylate target proteins carrying the biotin ligase recognition peptide | ACT2 | BASTA |
Quality Control Comments
There is no quality control data for this stock.