NASC stock number: N2107343
donor stock number: pUBQ10::2xmCITRINE-4K4Q-Farn (4+)
other name: 4K4Q-Farn
Resource Type: seed
Availability: available
Donors:- Yvon Jaillais
Donation Date: 01/17/2020
Date Released: 03/13/2020
Description:
Transgenic surface charge biosensor line (4+ charge). Expresses an mCITRINE (cYFP) fluorescent protein fused to a carboxy (C)-terminal farnesyl anchor in conjunction with an adjacent unstructured peptide (net positive charge of 4). Col-0 plants were transformed by the floral dip method. BASTA resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
mCITRINE (cYFP) expression localized to the plasma membrane and endomembrane
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/nplants.2016.89 | 27322096 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pUBQ10::2xmCITRINE-4K4Q-Farn | mCITRINE (cYFP) fluorescent protein fused to a carboxy (C)-terminal farnesyl anchor in conjunction with an adjacent unstructured peptide (net positive charge of 4) | UBQ10 | mCITRINE | BASTA |
Quality Control Comments
There is no quality control data for this stock.