NASC stock number: N2109517
donor stock number: PAP1 in V111_25D
other name: PAP1 in V111
Resource Type: seed
Availability: available
Donors:- Peter Doerner
Donation Date: 09/13/2021
Date Released: 12/13/2021
Description:
Transgenic line. V111_25D homozygous transgenic plants (CS2109499) were transformed with the PAP1 plasmid construct by Agrobacterium using the floral dip method. T1 seeds were harvested and selected by Basta spraying. T2 seed was selected on MSO plates for 3:1 segregation. Resistant plants were transferred to a greenhouse for further growth. T3 seed was harvested and screened on MSO plates. Homozygous plants were transferred to a greenhouse and grown to produce a large volume of seed.
Growth Requirement: none
Marker: BASTA, kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Upon estradiol induction, expresses nuclear localised H2B-Venus fluorescence and PAP1 in root trichoblasts. PAP1 expression causes visible and fluorescent signal in trichoblasts caused by production of anthocyanins.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1111/tpj.14192 | 30548978 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pSHV2:XVE>H2B-Venus | estradiol-inducible H2B-Venus fluorescent fusion protein | pSHV2 | H2B-Venus | kanamycin |
| LexAop-PAP1 | plasmid construct | PAP1 | BASTA |
Quality Control Comments
There is no quality control data for this stock.