ABRC
Arabidopsis Biological Resource Center

Description:

ttm3-3 mutant generated using CRISPR-Cas9. The TTM3-specific sequence (sgTTM3) was cloned into the PTTK352 vector, containing Cas9 in a cassette with hygromycin resistance and red fluorescent protein (RFP) as selection markers. Arabidopsis Col-0 plants were transformed with pTTK352-sgTTM3. T1 generation plants were selected via hygromycin resistance. ttm3-3 was identified by PCR followed by sequencing. In the T2 generation, seeds not expressing RFP (lacking Cas9) were selected. The resulting ttm3-3 allele harbours a 16 base-pair deletion in the TTM3 coding sequence that abolishes TTM3 protein accumulation and also reduces TTM3 transcript levels in planta.

Growth Requirement: none

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Publications