Name / Stock Number: CS2109802

View Order History

NASC stock number: N2109802

donor stock number: rbcs1a2b3b BB3

other name: BigBoi 3

Resource Type: seed

Availability: not available

Donors:
  • Alistair McCormick

Donation Date: 09/13/2021

Date Released: 10/12/2022

Description:

rbcs1a2b3b triple KO mutant (BigBoi) produced using CRISPR/Cas9. Based on the rbcs1a2b double mutant that was generated by crossing T-DNA insertion lines GABI_608F01 (CS458333) and GABI_324A03 (CS431011).

Growth Requirement: none

Marker: sulfadiazine

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT1G67090
  • RBCS1A
  • ribulose bisphosphate carboxylase small chain 1A
    		•  polymorphism SIGnAL TAIR ARAPORT
    AT5G38420
  • Rubisco small subunit 2B
  • RBCS2B
  • DEG24
    		•  polymorphism SIGnAL TAIR ARAPORT
    AT5G38410
  • Rubisco small subunit 3B
  • RBCS3B
    		•  polymorphism SIGnAL TAIR ARAPORT

    Germplasm

    2

    Very slow growth rate compared to WT, with pale leaves. The life cycle is 4-5 months and has very low seed yield (~10% of WT).

    Polymorphisms

    Polymorphism Mutagen Genotype Inheritance Gene Evidence attribution
    rbcs1a-1 T-DNA insertion homozygous
  • AT1G67090
    		• donor
    rbcs2b-1 T-DNA insertion homozygous
  • AT5G38420
    		• donor
    rbcs3b-7 CRISPR homozygous
  • AT5G38410
    		• donor

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1093/jxb/eraa316 32734287
    Transgene Description Promoter Reporter Marker
    pAC161 T-DNA sulfadiazine

    Quality Control Comments

    Add your quality control comment here

    There is no quality control data for this stock.