Name / Stock Number: CS2109987

other name: vih2-4 + VIH1(amiRNA) #3

donor stock number: pMDC7::amiRNA-VIH1/vih2-4, #3, T4-4, 20190916

NASC stock number: N2109987

Resource Type: seed

Availability: available

Donors:
  • Jinsheng Zhu
  • Michael Hothorn

Donation Date: 09/13/2021

Date Released: 04/26/2022

Description:

vih2-4 mutant expressing an estradiol-inducible artificial microRNA (amiRNA) targeting the VIH1 transcript. Fragments containing AtVIH1(amiRNA) were amplified and introduced into the pMDC7 binary vector. Plants were transformed by the floral dip method. Hygromycin resistant. Homozygous for the transgene.

Growth Requirement: none

Marker: hygromycin, sulfadiazine

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104

Germplasm

2

smaller rosette; dwarf

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.7554/eLife.43582 31436531
Transgene Description Promoter Reporter Marker
pAC161 T-DNA sulfadiazine
pMDC7::amiRNA-VIH1 estradiol-inducible amiRNA construct targeting VIH1 hygromycin

Quality Control Comments

There is no quality control data for this stock.