NASC stock number: N2109987
donor stock number: pMDC7::amiRNA-VIH1/vih2-4, #3, T4-4, 20190916
other name: vih2-4 + VIH1(amiRNA) #3
Resource Type: seed
Availability: available
Donors:- Jinsheng Zhu
- Michael Hothorn
Donation Date: 09/13/2021
Date Released: 04/26/2022
Description:
vih2-4 mutant expressing an estradiol-inducible artificial microRNA (amiRNA) targeting the VIH1 transcript. Fragments containing AtVIH1(amiRNA) were amplified and introduced into the pMDC7 binary vector. Plants were transformed by the floral dip method. Hygromycin resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: hygromycin, sulfadiazine
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
smaller rosette; dwarf
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.7554/eLife.43582 | 31436531 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pAC161 | T-DNA | sulfadiazine | ||
| pMDC7::amiRNA-VIH1 | estradiol-inducible amiRNA construct targeting VIH1 | hygromycin |
Quality Control Comments
There is no quality control data for this stock.