NASC stock number: N2109994
donor stock number: pVIH2::VIH2-KD/AA-mCit/vih1(+/-) vih2(-/-), T3
other name: vih1-2 (+/-) vih2-4 + VIH2(KD/AA)-mCit
Resource Type: seed
Availability: available
Donors:- Jinsheng Zhu
- Michael Hothorn
Donation Date: 09/13/2021
Date Released: 04/26/2022
Description:
vih1-2 (+/-) vih2-4 double mutant expressing VIH2 containing point mutations in the kinase domain (VIH2(KD/AA)). Plants were transformed by the floral dip method. Hygromycin resistant.
Growth Requirement: none
Marker: kanamycin, sulfadiazine, hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
seedling lethal
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.7554/eLife.43582 | 31436531 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pAC161 | T-DNA | sulfadiazine | ||
| pROK2 | T-DNA | kanamycin | ||
| pVIH2::VIH2-KD/AA-mCit | mCitrine-tagged kinase domain-mutated VIH2 expressed from the VIH2 promoter | pVIH2 | mCitrine | hygromycin |
Quality Control Comments
There is no quality control data for this stock.