Name / Stock Number: CS2110119

NASC stock number: N2110119

donor stock number: SRS7pro::GUS

Resource Type: seed

Availability: available

Donors:
  • Eva Sundberg

Donation Date: 09/13/2021

Date Released: 06/28/2022

Description:

Transgenic reporter line. Genomic DNA was isolated from A. thaliana floral buds and used to amplify the sequence immediately upstream of the translational start site from SRS7 (1.9 kb). Purified fragment was transferred by Gateway recombination into pGWB3 and the plasmid was transferred to Rhizobium radiobacter strain C58C1 by the freeze-thaw method and transformed to Arabidopsis. Homozygous line was selected using the kanamycin resistance marker and genotyping was performed by PCR.

Growth Requirement: none

Marker: kanamycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.3389/fpls.2018.00150 29491878
Transgene Description Promoter Reporter Marker
SRS7pro::GUS GUS reporter gene construct SRS7pro GUS kanamycin

Quality Control Comments

There is no quality control data for this stock.