NASC stock number: N2110785
donor stock number: iDePP UBQ10prom::GVG::6UAS::MAP-mCHERRY-dOCRL
other name: MAP-mCH-dOCRL
Resource Type: seed
Availability: available
Donors:- Yvon Jaillais
Donation Date: 09/13/2021
Date Released: 10/05/2021
Description:
Transgenic line expressing the iDePP system for the inducible depletion of PI(4,5)P2 in Arabidopsis. Consists of the catalytic domain of Drosophila OCRL (dOCRL, a 5-phosphatase), fused at its N-terminus to a membrane anchoring peptide (MAP, myristoylation and palmitoylation) and mCherry (mCH) for visualisation, with expression driven by a dexamethasone (dex)-inducible promoter. Col-0 plants were transformed by floral dipping. Hygromycin resistant. Homozygous for the transgene. This line is part of the iDePP set of lines (CS2110797).
Growth Requirement: induction upon dexamathasone (dex) treatment
Marker: hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
specific depletion of PI(4,5)P2 levels at the plasma membrane in living plant cells within a few hours of induction by dexamethasone (dex)
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/s41477-021-00907-z | 34007035 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| UBQ10prom::GVG::6UAS::MAP-mCHERRY-dOCRL | Drosophila melanogaster OCRL catalytic domain (168-509) fused at its N-terminus to a membrane anchoring peptide and mCherry, expression driven by a dexamethasone-inducible promoter | UBQ10 | mCherry | hygromycin |
Quality Control Comments
There is no quality control data for this stock.