NASC stock number: N2110786
donor stock number: iDePP control UBQ10prom::GVG::6UAS::MAP-mCHERRY-dOCRL(dead)
other name: MAP-mCH-dOCRLdead
Resource Type: seed
Availability: available
Donors:- Yvon Jaillais
Donation Date: 09/13/2021
Date Released: 10/05/2021
Description:
Transgenic control line for iDePP, a system for the inducible depletion of PI(4,5)P2 in Arabidopsis, made catalytically inactive by site-directed D468G mutation in the Drosophila OCRL (dOCRL) catalytic domain. Col-0 plants were transformed by floral dipping. Hygromycin resistant. Homozygous for the transgene. This line is part of the iDePP set of lines (CS2110797).
Growth Requirement: induction upon dexamathasone (dex) treatment
Marker: hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/s41477-021-00907-z | 34007035 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| UBQ10prom::GVG::6UAS::MAP-mCHERRY-dOCRL(dead) | Drosophila melanogaster OCRL catalytic domain (168-509), made catalytically dead with the D468G mutation, fused at its N-terminus to a membrane achoring peptide and mCherry for visualization | UBQ10 | mCherry | hygromycin |
Quality Control Comments
There is no quality control data for this stock.