Name / Stock Number: CS2110791

NASC stock number: N2110791

donor stock number: iDePP x PI(4,5)P2 marker (TUBBYc)

other name: MAP-mCH-dOCRL x mCit-TUBBY

Resource Type: seed

Availability: available

Donors:
  • Yvon Jaillais

Donation Date: 09/13/2021

Date Released: 10/05/2021

Description:

Transgenic line generated from a cross between the iDePP transgenic line (CS2110785) and the PI(4,5)P2 biosensor mCit-TUBBYc (CS2105610). Allows evaluation of the dynamics and cell type specificity of PI(4,5)P2 removal when using iDePP. Col-0 background. Hygromycin and BASTA resistant. Homozygous for the transgenes. This line is part of the iDePP set of lines (CS2110797).

Growth Requirement: induction upon dexamathasone (dex) treatment

Marker: BASTA, hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Induction of MAP-mCH-dOCRL (iDePP) releases fluorescence of the PI(4,5)P2 biosensor mCit-TUBBYc from the plasma membrane.

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1038/s41477-021-00907-z 34007035
Transgene Description Promoter Reporter Marker
UBQ10prom::CITRINE-1xTUBBY-C(TUBBY) UBQ10 mCITRINE BASTA
UBQ10prom::GVG::6UAS::MAP-mCHERRY-dOCRL Drosophila melanogaster OCRL catalytic domain (168-509) fused at its N-terminus to a membrane anchoring peptide and mCherry, expression driven by a dexamethasone-inducible promoter UBQ10 mCherry hygromycin

Quality Control Comments

There is no quality control data for this stock.