NASC stock number: N2110850
donor stock number: de23 HE2 #25
other name: ect2-1/ect3-1/3xHA-ECT2 L2
Resource Type: seed
Availability: available
Donors:- Laura Arribas Hernandez
- Peter Brodersen
Donation Date: 08/30/2021
Date Released: 09/27/2021
Description:
Complementation line. ect2-1/ect3-1 (de23) mutant (CS2110128) expressing the 3xHA-ECT2 protein fusion under the control of the corresponding endogenous ECT2 promoter (ECT2p:3xHA-ECT2-ECT2t). de23 mutant plants were transformed by floral dipping. BASTA resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: BASTA, kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
complementation of the de23 mutant (CS2110128) phenotype
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.17.00833 | 29643069 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin | ||
| ECT2p:3xHA-ECT2-ECT2t | 3xHA-ECT2 protein fusion with the native ECT2 terminator driven by the ECT2 native promoter | ECT2 | 3xHA | BASTA |
Quality Control Comments
There is no quality control data for this stock.