NASC stock number: N2110852
donor stock number: de23 FE3 #30
other name: ect2-1/ect3-1/FLAG-ECT3 L2
Resource Type: seed
Availability: available
Donors:- Laura Arribas Hernandez
- Peter Brodersen
Donation Date: 08/30/2021
Date Released: 09/27/2021
Description:
Complementation line. ect2-1/ect3-1 (de23) mutant (CS2110128) expressing the FLAG-ECT3 protein fusion under the control of the corresponding endogenous ECT3 promoter (ECT3p:FLAG-ECT3-ECT3t). de23 mutant plants were transformed by floral dipping. BASTA resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: BASTA, kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
complementation of the de23 mutant (CS2110128) phenotype
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.17.00833 | 29643069 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin | ||
| ECT3p:FLAG-ECT3-ECT3t | FLAG-ECT3 protein fusion with the native ECT3 terminator driven by the ECT3 native promoter | ECT3p | FLAG | BASTA |
Quality Control Comments
There is no quality control data for this stock.