Arabidopsis Biological Resource Center

Description:

Set of 400 recombinant inbred lines (RIL). The population was generated from accessions Hi-0 and Tsu-0, which have opposite phenotypes for shoot branching plasticity in response to nitrate. Higher plasticity line Tsu-0, grown under nitrate sufficient conditions, produces more branching than those grown under nitrate deficient conditions. Lower plasticity line Hi-0 produces similar numbers of branches, regardless of nitrate supply. The initial F1 was generated by reciprocal crossing, using each accession as both a male and a female parent. F2s were generated by selfing individuals from each reciprocal F1 cross. 200 individuals from each cross direction (400 total) were picked for further rounds of selfing. In each round, a single seed was grown to generate the next generation. This process was repeated up to generation F8 (i.e. 6 rounds of single-seed-descent selfing after the initial F2 segregating population). The direction of the cross is indicated by the RIL name, where the female parent is indicated first (for example, Hi-0xTsu-0.030 is individual 30 from a cross using Hi-0 as a female parent). For most mapping purposes, researchers can ignore the cross direction; but this information may be of interest to study epistatic interactions between mitochondrial and nuclear genomes or segregation distortion. F8 seed donated, advanced one generation at NASC.

Growth Requirement: none

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ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $1960 / $15680