NASC stock number: N23886
other name: twn1-1
donor stock number: twin (twn); twin1 (twn1); emb214
Resource Type: seed
Availability: available
Donors:- Daniel Vernon
Donation Date: 09/30/2009
Date Released: 05/31/2010
Description:
twn1 seeds were from a large collection of embryo-defective mutants identified by the Meinke laboratory (Errampalli et al., Plant Cell, 1991), from the K. Feldmann T-DNA collection. Does not contain a functional T-DNA insert, no antibiotic resistance. Submitted seeds obtained from a selfed twn1 homozygote, previously backcrossed more than once to Ws.
Growth Requirement: germinate on agar plates with MS media + 1% sucrose, to observe germination of small secondary (twin) seedlings
Marker:
Background: Ws (Wassilewskija)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Incomplete penetrance and highly variable phenotypein mutant embryos. About 8 % of seeds contain embryos that display suspensor proliferation or polyembryony; Some twin embryos are viable and germinate. In addition to twinning, some embryos show variable defects in the embryo proper early in embryogenesis. Cotyledon defects are present in about 10% of embryos/seedlings and occur independent of twinning. Defects in cotyledons/leaf organization do not extend into post-embryonic development beyond the first leaf pair. Mutant phenotype(s) appears to be embryo-specific.
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| 3850:1003 | T-DNA | kanamycin |
Quality Control Comments
There is no quality control data for this stock.