donor stock number: SL40
NASC stock number: N25171
Resource Type: seed
Availability: available
Donors:- Bindu Nishal
- Titima Tantikanjana
- Venkatesan Sundaresan
Donation Date: 07/20/2005
Date Released: 07/29/2005
Description:
Line generated by the system of inducible insertional mutagenesis based on the Ac-Ds family of transposons for targeted tagging in Arabidopsis; the Ac and Ds elements were placed in the same T-DNA construct and a heat shock-inducible transposase fusion was utilized to control the levels of transposase gene expression, generating a transposition that can be subsequently stabilized without requiring crossing or segregation; mapped by thermal asymmetric interlaced-PCR; can be used as potential launch pad for heat shock mutagenesis; Kanamycin resistant (50 mg/L).
Growth Requirement: none
Marker: kanamycin
Background: Ws (Wassilewskija)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1104/pp.104.050633 | 15644463 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pYS11 | heat shock inducible transposon/transposase construct; can be used as potential launch pad for heat shock mutagenesis; streptomycin (excision marker), kanamycin (re-insertion marker) | Glycine max HSP (controlling transposase), CaMV35S | GUS | kanamycin |
Quality Control Comments
There is no quality control data for this stock.