Name / Stock Number: CS29914

NASC stock number: N29914

donor stock number: 3ksi-2

Resource Type: seed

Availability: available

Donors:
  • Georg Jander

Donation Date: 04/29/2024

Date Released: 05/09/2024

Description:

CRISPR/Cas9 generated knockout of EcCYP87A126, the cytochrome P450 responsible for sterol side chain cleavage and the initiation of cardenolide biosynthesis in Erysimum cheiranthoides. One bp insertion in coding sequence of CYP87A126 (Erche04g002680) results in loss of function and complete loss of cardenolide accumulation in plant tissues. No obvious visual phenotype or special growth requirements.

Growth Requirement: Stratification for 2 days at 4C recommended

Marker:

Background: Elbtalaue

ABRC Comment:

Format Shipped: 15 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

5beta-cardenolide synthesis reduced, accumulation of delta5-cardenolides

Erysimum cheiranthoides 330168

Additional Information

DOI PubMed
10.1101/2024.04.10.588904 38645095

Quality Control Comments

There is no quality control data for this stock.