Name / Stock Number: CS29917

NASC stock number: N29917

donor stock number: p5br2-1

Resource Type: seed

Availability: available

  • Georg Jander

Donation Date: 04/29/2024

Date Released: 05/09/2024


CRISPR/Cas9 generated knockout of EcCYP71B132, a cytochrome P450 that was identified as a candidate for cardenolide biosynthesis based on expression, but the knockout mutant has no cardenolide-related phenotype. One bp deletion in coding sequence of CYP71B132 (Erche08g004170) does not alter cardenolide profile. No obvious visual phenotype or special growth requirements. Other metabolome-related phenotypes are possible but have not been investigated.

Growth Requirement: Stratification for 2 days at 4C recommended


Background: Elbtalaue

ABRC Comment:

Format Shipped: 15 seeds per vial

Base / Commercial Price: $15 / $120



5beta-cardenolide synthesis eliminated, accumulation of 5alpha-cardenolides, delta4-cardenolides, and delta5-cardenolides

Erysimum cheiranthoides 330168

Additional Information

DOI PubMed
10.1101/2024.04.10.588904 38645095

Quality Control Comments

There is no quality control data for this stock.