Name / Stock Number: CS29920

NASC stock number: N29920

donor stock number: card5-2

Resource Type: seed

Availability: available

Donors:
  • Georg Jander

Donation Date: 04/29/2024

Date Released: 05/09/2024

Description:

CRISPR/Cas9 generated knockout of EcCYP71B132, a cytochrome P450 that was identified as a candidate for cardenolide biosynthesis based on expression, but the knockout mutant has no cardenolide-related phenotype. One bp deletion in coding sequence of CYP71B132 (Erche08g004170) does not alter cardenolide profile. No obvious visual phenotype or special growth requirements. Other metabolome-related phenotypes are possible but have not been investigated.

Growth Requirement: Stratification for 2 days at 4C recommended

Marker:

Background: Elbtalaue

ABRC Comment:

Format Shipped: 15 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

cardenolide biosynthesis eliminated; accumulation of pregnane intermediates

Erysimum cheiranthoides 330168

Additional Information

Quality Control Comments

There is no quality control data for this stock.