NASC stock number: N29920
donor stock number: card5-2
Resource Type: seed
Availability: available
Donors:- Georg Jander
Donation Date: 04/29/2024
Date Released: 05/09/2024
Description:
CRISPR/Cas9 generated knockout of EcCYP71B132, a cytochrome P450 that was identified as a candidate for cardenolide biosynthesis based on expression, but the knockout mutant has no cardenolide-related phenotype. One bp deletion in coding sequence of CYP71B132 (Erche08g004170) does not alter cardenolide profile. No obvious visual phenotype or special growth requirements. Other metabolome-related phenotypes are possible but have not been investigated.
Growth Requirement: Stratification for 2 days at 4C recommended
Marker:
Background: Elbtalaue
ABRC Comment:
Format Shipped: 15 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
cardenolide biosynthesis eliminated; accumulation of pregnane intermediates
Erysimum cheiranthoides 330168
Additional Information
Quality Control Comments
There is no quality control data for this stock.