donor stock number: T-FGC231B
NASC stock number: N3967
Resource Type: seed
Availability: available
Donors:- Ed Butler
- Chromatin Functional Genomics Consortium
Donation Date: 11/06/2001
Date Released: 02/28/2002
Description:
Carries a dominant negative transgene at the PKL locus; Generated by Agrobacterium-mediated transformation using the binary construct pFGC2315; single-insertion event (determined by Southern analysis); potentially confers dominant negative silencing of target gene, as well as dominant hygromycin resistance marker; specific reduction of target mRNA as evidenced by RT-PCR; Gene product is a chromatin remodeling complex subunit A (Swi2/Snf2 homologs), ATP-dependent DNA helicase (EC:3.6.1.3); other symbols used for this Gene: CHA6 (chromatin remodeling complex A), and GYMNOS.
Growth Requirement: none
Marker: hygromycin
Background: Ws (Wassilewskija)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
homozygous
dsRNA silencing
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pFGC2315 | RNAi construct | hygromycin |
Quality Control Comments
There is no quality control data for this stock.