Name / Stock Number: CS3995

donor stock number: T-FGC524A

NASC stock number: N3995

Resource Type: seed

Availability: available

Donors:
  • Ed Butler
  • Chromatin Functional Genomics Consortium

Donation Date: 03/20/2002

Date Released: 04/03/2002

Description:

Carries a dominant negative transgene at the SGA1 locus; generated by Agrobacterium-mediated transformation using the binary construct pFGC5240; single-insertion event (determined by Southern analysis); potentially confers dominant negative silencing of target gene, as well as dominant hygromycin resistance marker; specific reduction of target mRNA as evidenced by RT-PCR; gene product is homologous to yeast ASF1 (anti-silencing factor), silencing gene A group (ScASF1 like).

Growth Requirement: none

Marker: hygromycin

Background: Ws (Wassilewskija)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104

Germplasm

2

homozygous

dsRNA silencing

Arabidopsis thaliana 3702

Additional Information

Transgene Description Promoter Reporter Marker
pFGC5240 RNAi construct hygromycin

Quality Control Comments

There is no quality control data for this stock.