donor stock number: 307
NASC stock number: N4932
Resource Type: seed
Availability: available
Donors:- Patrick Gallois
Donation Date: 02/22/2000
Date Released: 02/22/2000
Description:
single line of a transgenic, promoter trap population which contains the promoterless pDGus Bin19 construct from Dr. Keith Lindsey; a promoterless GUS with its ATG next to the T-DNA left border; kanamycin resistant. plants of C24 were transformed by co-cultivation of root explants with Agrobacterium tumefaciens followed by selection on kanamycin; seeds were harvested from individual primary transformants (T1); each progeny was germinated on kanamycin and resistant seedlings transferred to soil; distribution stocks are T3 or T4 generations bulk propagated from single primary transformants; more than 50% of the lines express GUS in at least one tissue; all lines have been screened for GUS expression in roots and for wavy root mutations by P.H. Masson.
Growth Requirement: none
Marker: kanamycin
Background: C24
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
T-DNA insertion
Arabidopsis thaliana 3702
Additional Information
DOI | PubMed |
---|---|
10.1046/j.1365-313x.1998.00279.x | 9839461 |
Transgene | Description | Promoter | Reporter | Marker |
---|---|---|---|---|
pDGus Bin19 | promoter trap T-DNA | GUS | kanamycin |
Quality Control Comments
There is no quality control data for this stock.