other name: FLC2RL
donor stock number: F-1
NASC stock number: N6545
Resource Type: seed
Availability: available
Donors:- Kiyoshi Onai
- Masahiro Ishiura
Donation Date: 04/29/2005
Date Released: 05/17/2005
Description:
Transgenic line containing both FT::LUC+ and CAB2::Rluc reporter genes in the same locus; constructed reporter genes were made by fusing the promoter of a clock-regulated Arabidopsis flowering-time gene FLOWERING LOCUS T (FT) to a coding region of a modified firefly luciferase gene (LUC+); pFT::LUC+ reporter gene cassette was inserted just upstream of a pCAB2::Rluc gene cassette of binary vector pBIC2RL, giving pBIFLC2RL; pBIC2RL carried the pBI101 backbone and the pCAB2::Rluc gene casssette in place of both the beta-glucuronidase coding region (GUS) and the transcriptional terminator sequence of the Agrobacterium nopaline synthase gene (T NOS); kanamycin resistant.
Growth Requirement: kanamycin resistant
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1111/j.1365-313X.2004.02191.x | 15361136 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| FT::LUC+/CAB2::Rluc | promoter of FLOWERING LOCUS T (FT) fused to coding region of modified firefly luciferase gene (LUC+) inserted just upstream of a pCAB2::Rluc gene cassette of binary vector pBIC2RL | FLOWERING LOCUS T (FT), CAB2 | LUC | kanamycin |
Quality Control Comments
There is no quality control data for this stock.