NASC stock number: N66315
donor stock number: pPIN1::PIN1-GFP; pSTM::STM-VENUS; pRIBO::2xCFP-N7
Resource Type: seed
Availability: available
Donors:- Elliot Meyerowitz
Donation Date: 12/09/2010
Date Released: 01/18/2011
Description:
transgenic line expressing pPIN1::PIN1-GFP, pSTM::STM-VENUS, and pRIBO::2xCFP-N7 constructs
Growth Requirement: Kanamycin resistant for pPIN1::PIN1-GFP; Basta resistant for pSTM::STM-VENUS; Gentamycin resistant for pRIBO::2XCFP-N7
Marker:
Background: Ler (Landsberg erecta)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1242/dev.010298 | 17827180 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pPIN1::PIN1-GFP | mGFP5 inserted after the PIN1 gene (3506 bp upstream of the ATG to 2181 bp downstream of the stop codon) | pPIN1 3506 bp upstream of the ATG | GFP | kanamycin |
| pRIBO::2XCFP-N7 | 2.6 kb promoter sequence of 60S ribosomal protein L2 gene was fused to 2 copies of eCFP followed by N7 nuclear localization sequence | EMB2296 2.6 kb | 2XCFP | gentamycin |
| pSTM::STM-VENUS | 5.76 kb of STM upstream regulatory sequence, STM cDNA, VENUS reporter | BASTA |
Quality Control Comments
There is no quality control data for this stock.