NASC stock number: N66319
donor stock number: pPIN1::PIN1-GFP; pSTM::STM-YFP; pWUS::DsRed-N7
Resource Type: seed
Availability: available, formerly distributed as CS23899
Donors:- Elliot Meyerowitz
Donation Date: 12/09/2010
Date Released: 08/05/2011
Description:
transgenic line carrying pPIN1::PIN1-GFP, pSTM::STM-YFP, and pWUS::dsRed-N7 constructs
Growth Requirement: none
Marker:
Background: Ler (Landsberg erecta)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1242/dev.010298 | 17827180 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pPIN1::PIN1-GFP | mGFP5 inserted after the PIN1 gene (3506 bp upstream of the ATG to 2181 bp downstream of the stop codon) | pPIN1 3506 bp upstream of the ATG | GFP | kanamycin |
| pWUS::dsRED-N7 | 4.4 kb of upstream and 1.5 kb of downstream WUS genomic regulatory sequencs separated by the DsRed coding region fused to the N7 NLS | WUS | dsRED-N7 | gentamycin |
| pSTM::STM-YFP | pSTM | YFP |
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| Researcher reported a problem with germination | user | 02/28/2022 | To view detailed data, click here |
| Germination was found to be 90%, which is within the acceptable range for germination percentage (81-100%) set by ABRC. | ABRC | 03/21/2022 | To view detailed data, click here |