NASC stock number: N66494
donor stock number: GL3::GL3-YFP
Resource Type: seed
Availability: available
Donors:- John Schiefelbein
Donation Date: 01/12/2011
Date Released: 03/17/2011
Description:
Transgenic line; the EYFP-coding region was amplified from pEYFP (Clontech) and fused in-frame to the 3' end of a GL3 genomic DNA fragment containing the entire GL3 gene, including ~1 kb upstream of the start codon and 1 kb downstream of the stop codon; the GL3::GL3-YFP construct was sub-cloned into the BglII site of the T-DNA vector pAL47 (Lloyd and Davis, 1994) for plant transformation. Genotype for transgene is unknown.
Growth Requirement: Kanamycin resistant.
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
the maximum GUS activity is observed in the meristematic region of the developing root tip; significantly higher levels of GUS activity in cells located in the H position than the N position
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1242/dev.01565 | 15590742 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| GL3::GL3-YFP | EYFP-coding region fused in-frame to the 3' end of a GL3 genomic DNA fragment containing the entire GL3 gene, including ~1 kb upstream of the start codon and 1 kb downstream of the stop codon | GL3 | YFP | kanamycin |
Quality Control Comments
There is no quality control data for this stock.