NASC stock number: N67157
other name: #289
donor stock number: pAG::LhG4 pOp(6x)::HF-RPL18 35S::AP1-GR ap1-1 cal1-1
Resource Type: seed
Availability: available
Donors:- Elliot Meyerowitz
Donation Date: 01/18/2012
Date Released: 02/22/2012
Description:
ap1-1 cal1-1 plants (CS6161) were sequentially transformed with 35S::AP1-GR (Yu et al., Nat Genetics 2004) and pOp(6x)::HF-RPL18 constructs. The His- and FLAG-tagged RPL18 (HF-RPL18) coding sequence was isolated from pGATA 35S::HF-RPL18 (Zanetti et al, 2005) and inserted into the pV-TOP vector under the control of pOp(6x) promoter (Craft et al., Plant J 2005). The double transgenic line 35S::AP1-GR pOp(6x)::HF-RPL18 ap1-1 cal1-1, carrying Kanamycin and Hygromycin resistance, was crossed to a Basta-resistant pAG::LhG4 line homozygous for ap1-1, cal1-1. Genotype for transgene is unknown.
Growth Requirement: double transgenic (kanamycin and hygromycin resistant) crossed with single transgenic (basta resistant)
Marker: BASTA, hygromycin, kanamycin
Background: Ler (Landsberg erecta)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Indistinguishable from ap1-1 cal1-1 double mutant. Upon DEX treatment, synchronized floral meristem formation is induced.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/msb.2010.76 | 20924354 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| 35S::AP1-GR | kanamycin | |||
| pOp(6x)::HF-RPL18 | His- and FLAG-tagged RPL18 (HF-RPL18) coding sequence under the control of pOp(6x) promoter | pOp(6x) | hygromycin | |
| pAG::LhG4 | AG | BASTA, hygromycin, kanamycin |
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| Germination was found to be 90%, which is within the acceptable range for germination percentage (81-100%) set by ABRC. | ABRC | 09/20/2018 | To view detailed data, click here |
| Researcher reported low germination | ABRC | 08/22/2017 | To view detailed data, click here |