NASC stock number: N67829
donor stock number: SC-GFP + VN-RFP
Resource Type: seed
Availability: available
Donors:- Richard Slotkin
Donation Date: 06/08/2012
Date Released: 06/19/2012
Description:
Constructed for pollen nuclei visualization and cell sorting of specific pollen nuclei; wt Col homozygous for two separate transgenes: the MGH3 promoter driving the MGH3 protein coding region, fused to mGFP in the vector backbone pMDC107 (HygroR in plants), and the Actin 11 promoter driving a fusion protein of histone H2B and mRFP (KanR in plants)
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
GFP is apparent in bicellular pollen in the generative cell nucleus, and in mature pollen and in the pollen tube in the sperm cell nuclei; RFP is apparent in bicellular pollen in the generative cell nuclei, and in mature pollen in the vegetative nucleus
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| MGH3:mGFP | MGH3 promoter driving the MGH3 protein coding region, fused to mGFP | MGH3 | mGFP | hygromycin |
| Actin11:HISH2B:mRFP | Actin 11 promoter driving a fusion protein of histone H2B and mRFP | Actin11 | mRFP | kanamycin |
Quality Control Comments
There is no quality control data for this stock.