NASC stock number: N67830
donor stock number: GFP:MAP65-1
Resource Type: seed
Availability: available
Donors:- Jessica Lucas
- Sidney Shaw
Donation Date: 06/22/2012
Date Released: 08/21/2012
Description:
Transgenic line; MAP65-1 genomic DNA was PCR amplified from Col-0 DNA and inserted into pPZP211. eGFP sequence was amplified without the stop codon and cloned upstream of MAP65-1 gene in pPZP211 vector. 2.6 kb upstream of MAP65-1 gene was inserted upstream of eGFP to drive gene expression. Agrobacterium strain GV3001 was used to transform map65-1-2 homozygous mutant line, derived from SALK_118225. T1 seeds were selected on Kanamycin. Plants are segregating for the transgene.
Growth Requirement: select on MS plates containing kanamycin
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.111.084970 | 21551389 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| GFP:MAP65-1 | MAP65-1 | GFP | kanamycin |
Quality Control Comments
There is no quality control data for this stock.