ABRC
NASC stock number: N68649
donor stock number: Col-0 UBQ10:NTF::ACT2:BirA #1
Resource Type: seed
Availability: available
Donors:- Christine Queitsch
- Jennifer Nemhauser
Donation Date: 12/19/2013
Date Released: 03/06/2014
Description:
To create a transgenic line in which nearly all nuclei can be captured with the INTACT method, we transformed A. thaliana accession Col-0 with a construct (UBQ10:NTF) in which the UBQ10 promoter was fused with the NTF segment used in the original INTACT lines. We simultaneously transformed the UBQ10:NTF construct and the ACT2:BirA construct by floral dip to obtain a stable homozygous UBQ10:NTF::ACT2:BirA line. The UBQ10:NTF construct confers Kanamycin resistance at 50 ug/mL for plated seedlings; the ACT2:BirA construct confers BASTA resistance at 15 uM for plated seedlings.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
RFP expression on nuclear envelope
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| 10.1038/nprot.2010.175 | 21212783 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| UBQ10:NTF | UBQ10 promoter was fused with the NTF segment used in the original INTACT lines | UBQ10 | kanamycin | |
| ACT2:BirA | ACTIN2 promoter: from -1444 to -1 relative to ATG start codon driving the E. coli biotin ligase (BirA) gene, will biotinylate target proteins carrying the biotin ligase recognition peptide | ACT2 | BASTA |
Quality Control Comments
There is no quality control data for this stock.