donor stock number: vip4-1
NASC stock number: N68978
other name: vernalization independence 4
other name: vip4
Resource Type: seed
Availability: available
Donors:- Steve Van Nocker
Donation Date: 03/27/2015
Date Released: 05/05/2015
Description:
Insertional mutagen into introgression line Col:FRI (obtained from Rick Amasino) using vector pPZP201 modified to contain a gene for resistance to glufosinate. Insertion is within 10th (of 11) exon. Mutant was isolated from a population of T-DNA-mutagenized plants in a screen for plants with a cold-independent, early flowering phenotype. Mutant was backcrossed 5 times to Col-0 with final selection by progeny testing. Mutant retains resistance to glufosinate.
Growth Requirement: Homozygotes have reduced sterility at typical growth temperatures (22-24C) and above.
Marker:
Background: Col (Columbia)
ABRC Comment: In publications involving this stock, please acknowledge the donor and ABRC. Example template: vip4-1 was a gift from Steve van Nocker, and is made available through the Arabidopsis Biological Resource Center (CS68978).
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Phenotypes for homozygous vip4-1 mutants after 2-5 day stratification are the following: slightly early flowering due to repression of FLC; slight floral abnormality with sepals fringed with white and failing to enclose floral bud; variable fertility under standard growth conditions. Phenotypes are increasingly severe when plants are grown at higher temperatures (above 22 C).
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1046/j.1365-313X.2002.01380.x | 12207655 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pPZP201:BAR | T-DNA | CaMV 35S | BASTA |
Quality Control Comments
There is no quality control data for this stock.