NASC stock number: N69047
donor stock number: axr1-3 EBS:GUS
Resource Type: seed
Availability: available
Donors:- Anna Stepanova
- Joseph Ecker
Donation Date: 09/09/2015
Date Released: 09/11/2015
Description:
Transgenic line; This line carries an ethylene-inducible reporter EBS:GUS. EBS:GUS consists of a tandem of 5 direct repeats of the EIN3-binding site (cctcatgatCAAAGGGGGGATGCACTatttaa) from the promoter of a direct EIN3 target gene, EDF1 (AT1G25560), fused to the minimal (-46) 35S promoter and subcloned upstream of GUS in the pCAMBIA1381Z vector. Hygromycin resistant. Homozygous for the transgene. Homozygous for the axr1-3 mutation.
Growth Requirement: none
Marker: hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
GUS expression at root tip under alkaline stress conditions.
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| EBS:GUS | 5 direct repeats of the EIN3-binding site (cctcatgatCAAAGGGGGGATGCACTatttaa) from the promoter of a direct EIN3 target gene, EDF1 (AT1G25560), fused to the minimal (-46) 35S promoter and subcloned upstream of GUS | EBS | GUS | hygromycin |
Quality Control Comments
There is no quality control data for this stock.