donor stock number: nai2-1 GFP-h
NASC stock number: N69076
Resource Type: seed
Availability: available
Donors:- Mikio Nishimura
Donation Date: 12/15/2015
Date Released: 03/08/2016
Description:
nai2-1 contains a large genome deletion that includes At3g15940, At3g15950, At3g15960, At3g15970, and At3g15980. nai2-1 was isolated from a population of activation-tagged GFP-h plants in a screen for ER-body deficient mutants. The activation-tagging vector is pPCVICEn4HPT. GFP-h is an Arabidopsis (ecotype Col) transformed with a pro35S:SP-GFP-HDEL gene encoding SP-GFP-HDEL, which is composed of the signal peptide of pumpkin (Cucurbita maxima) 2S albumin, and GFP followed by a 12 amino-acid sequence including an ER-retention signal, HDEL. The construct was inserted into the pBI121 binary vector. Homozygous for the mutation. Homozygous for the transgene.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
ER body deficient mutant. ER bodies are hardly detected in seedlings and roots. The plants do not accumulate NAI2 mRNA and protein.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.108.059345 | 18780803 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pro35S:SP-GFP-HDEL | signal peptide of pumpkin (Cucurbita maxima) 2S albumin, and GFP followed by a 12 amino-acid sequence including an ER-retention signal, HDEL under the control of the CMV 35S promoter | CaMV 35S | GFP | kanamycin |
| pPCVICEn4HPT | activation tagging T-DNA containing signal peptide of pumpkin (Cucurbita maxima) 2S albumin, and GFP followed by a 12 amino-acid sequence including an ER-retention signal, HDEL | hygromycin |
Quality Control Comments
There is no quality control data for this stock.