donor stock number: pKAT1:HF-GFP-RPL18
NASC stock number: N69099
other name: line 15-3-1
Resource Type: seed
Availability: available
Donors:- Julia Bailey-Serres
Donation Date: 12/17/2015
Date Released: 01/21/2016
Description:
Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene from p35S:HF-GFP-RPL18 (CD3-2403) was amplified and inserted into pGATA-HF-RPL18 (CD3-1629) to generate pGATA-HF-GFP-RPL18 (CD3-1630). The KAT1 promoter sequence was amplified, cloned into pENTR-D-TOPO and recombined into pGATA-HF-GFP-RPL18 (CD3-1630). The Col-0 plant was transformed using the floral dip method. Kanamycin resistant. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Growth Requirement: none
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pKAT1:HF-GFP-RPL18 | The KAT1 promoter sequence, TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3’UTR of the OSC gene | KAT1 | His-FLAG, sGFP | kanamycin |
Quality Control Comments
There is no quality control data for this stock.