donor stock number: 35S::ATRGS1 hxk1-3 (line 7-7)
NASC stock number: N69142
other name: AtRGS1-YFP hxk1-3
other name: 35S::AtRGS1-YFP hxk1-3
Resource Type: seed
Availability: available
Donors:- Jianping Huang
- Alan Jones
Donation Date: 01/26/2016
Date Released: 04/01/2016
Description:
Transgenic line; AtRGS1 (encoding 1-459aa) was subcloned into pEarleyGate101, containing a 35S promoter and C-terminal YFP-HA and the hxk1-3 (CS69135) plant was transformed. Basta resistant. Homozygous for the transgene. Homozygous for the mutation (hxk1-3).
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Reduced growth in roots, leaf and stem length, rosette size and inflorescence. YFP expression localized to the plasma membrane without glucose, and endocytosed upon D-glucose treatment.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.3389/fpls.2015.00851 | 26528314 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| 35S::AtRGS1-YFP | 35S promoter, AtRGS1 (encoding 1-459aa), C-terminal YFP-HA | CaMV 35S | YFP | BASTA |
Quality Control Comments
There is no quality control data for this stock.