donor stock number: PCDKA;1:GUS rgs1-2
NASC stock number: N69152
Resource Type: seed
Availability: available
Donors:- Jianping Huang
- Alan Jones
Donation Date: 01/26/2016
Date Released: 04/01/2016
Description:
Transgenic line; 1.7 kb of genomic DNA containing the promoter region and the leader of the CDKA;1 gene was amplified and cloned into the pGUS1 vector upstream of the GUS coding sequence. The resulting cassette was subcloned into the SalI and ScaI restriction sites of pGSV4. The construct was crossed into rgs1-2 (CS69134) single mutant plants. Kanamycin resistant. Homozygous for the transgene. Homozygous for the mutation (rgs1-2).
Growth Requirement: none
Marker: kanamycin
Background:
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Longer roots and hypocotyls, larger rosette diameter and inflorescence. GUS expression in areas of active cell division: shoot and root meristems.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.3389/fpls.2015.00851 | 26528314 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| PCDKA;1:GUS | 1.7 kb of genomic DNA containing the promoter region and the leader of the CDKA;1 gene fused to GUS | CDKA;1 | GUS | kanamycin |
Quality Control Comments
There is no quality control data for this stock.