donor stock number: pTIR1:tir1E15K-GUS tir1-1 #3-5
NASC stock number: N69664
other name: MP2396
Resource Type: seed
Availability: available
Donors:- Mike Prigge
- Mark Estelle
Donation Date: 11/09/2016
Date Released: 02/01/2017
Description:
Transgenic line; TIR1 upstream region (-1978 to -13 bp) was fused to the tir1 cDNA harboring the E15K mutation and recombined into pMDC163 to create a translational GUS fusion. It was transformed into the tir1-1 (CS3798) background. Hygromycin resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Short roots, gravitropism defects, short inflorescence. Auxin resistant. Weaker than E12K mutant (CS69663).
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/nplants.2014.30 | 26236497 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pTIR1:tir1E15K-GUS | TIR1 upstream region (-1978 to -13 bp) fused to the tir1 cDNA harboring the E15K mutation fused to GUS | TIR1 | GUS | hygromycin |
Quality Control Comments
There is no quality control data for this stock.