donor stock number: icpk10,30
NASC stock number: N69946
other name: cpk10 cpk30 CPK10(M141G)-HA
Resource Type: seed
Availability: available
Donors:- Kun-Hsiang Liu
- Jen Sheen
Donation Date: 02/16/2018
Date Released: 03/26/2018
Description:
CPK10(M141G)-HA-NOS transgene in cpk10-1 cpk30-1 mutant background; 5.5-kb genomic fragment including the promoter region (3-kb) and coding region of CPK10 was amplified from genomic DNA, cloned into the plasmid HBT-HA-NOS, then mutagenized to generate pCPK10-CPK10(M141G)-HA-NOS. This construct was inserted into pCB302 and transformed into cpk10 cpk30/+ mutant plants by the floral dip method to obtain the 3MBiP-inducible icpk10,30 double mutant carrying the CPK10-CPK10(M141G)-HA-NOS transgene. Basta resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Embryo lethality of cpk10 cpk30 double mutant is rescued by the CPK10(M141G) gatekeeper residue inducible mutation, in which the CPK10 kinase function can be reversely inhibited by 3MBiP. Reduced expression of primary nitrate-responsive genes in the presence of 3MBiP, without displaying a visible phenotype in plant growth supported by nitrate.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1038/nature22077 | 28489820 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin | ||
| pCPK10-CPK10(M141G)-HA-NOS | 5.5-kb genomic fragment including the promoter region (3-kb) and coding region of CPK10 amplified from genomic DNA, cloned into the plasmid HBT-HA-NOS, then mutagenized to generate pCPK10-CPK10(M141G)-HA-NOS | CPK10 | BASTA |
Quality Control Comments
There is no quality control data for this stock.